![]() ![]() Forskolin (20 μM), an adenylate cyclase activator, was used for channel activation, and subsequently CFTRinh-172 (2 μM) for its inhibition. The mature CFTR 160-kDa band was present, and its localization at the surface membrane was confirmed. ![]() Ion currents were analyzed with patch clamp, and cell migration with the wound healing method. Here we tested the idea if CFTR is functionally expressed in BeWo cells, a trophoblastic cell line, and if it is involved in their migratory behavior.ĬFTR expression was studied in BeWo cells with RT-PCR, biotinylation and Western blot. In addition, ENaC and CFTR promote migration in placental trophoblastic cells and human airway cells, respectively. ENaC and CFTR are coexpressed in epithelia and have positive or negative functional interactions. ![]()
0 Comments
Leave a Reply. |
AuthorWrite something about yourself. No need to be fancy, just an overview. Archives
May 2023
Categories |